Tech Note: Culturing L929 Cell Line On A Thermo Scientific Nunc Nunclon Cell Culture Treated Surface

Thermo Scientific Nunc Nunclon cell culture products are tested for cell growth and plating efficiency using several different cell lines.

Nunc™ Nunclon™ products are tested with two cell lines L929, HEL 299 or F2002 and one Primary Chick Embryo cell culture for monolayer formation, plus cell line V79-4 for cloning efficiency.

L929 is a fibroblast-like cell line cloned from strain L.

The parent strain was derived from normal subartaneous areolar and adipose tissue of a male C3H/ An mouse.

This Tech Note describes a procedure for culturing L929 cell line on a Nunclon treated surface.