Tech Note: Culturing HEL 299 Cell Line On A Thermo Scientific Nunc Nunclon Cell Culture Treated SurfaceSource: Thermo Scientific Nalgene and Nunc
Thermo Scientific Nunc Nunclon cell culture products are tested for cell growth and plating efficiency using several different cell lines.
Nunc™ Nunclon™ products are tested with two cell lines L929, HEL 299 or F2002 and one Primary Chick Embryo cell culture for monolayer formation, plus cell line V79-4 for cloning efficiency.
The HEL 299 cell line is derived from embryonic lung tissue of a human male. It is a diploid fibroblast-like cell line initially developed for use in vaccine development.
This Tech Note describes a procedure for culturing HEL 299 cell line on a Nunclon treatedsurface.