BalanCD™ CHO Feed 1 BrochureSource: Irvine Scientific
BalanCD™ CHO Feed 1 is a chemically-defined protein-free feed medium designed to provide cell culture nutrients for fed-batch applications in conjunction with BalanCD™ CHO Growth A. The feed supplement has been developed to promote cell growth and MAb production in a variety of Chinese Hamster Ovary (CHO) cells. This formulation was developed using Ir vine Scientific's Rational Culture Media Design™ approach to carefully balance key nutrients that provide superior yields in CHO fed-batch applications.
BalanCD™ CHO Feed 1 is comprised solely of animal component-free, chemically defined components, contains no antibiotics or antimycotics, and has proven stability in both powder and liquid formats.
Product Handling & Storage
Product handle using aseptic techniques is required to avoid contamination. Do not use if cloudy or if solution has precipitated. Powder product should be stored dry at 2-8°C and in absence of light for up to 2 years. Liquid product should be stored at 2-8°C and in absence of light for up to 6 months. Extended stability studies for both powder and liquid are ongoing for this formulation.
The following products are available from Ir vine Scientific and developed for use in CHO fed-batch production systems. For additional information, please visit our website at www.ir vinesci.com or call Customer Service at (800) 427-5706.
These products are for RESEARCH USE only and not approved for human or veterinar y use, for application to humans or animals, or for use in clinical or in vitro procedures.
The study consisted of three model cell lines (CHO-S, CHO-M, and CHO-dhfr) grown in fed-batch mode using Irvine Scientific's BalanCD™ CHO Growth A and Feed 1 evaluated in a side by side comparative with several commercially available growth and feed media.The following culture parameters and experimental conditions applied to all:
- 10% (v/v) bolus feeds on days 1, 3, and 5
- Two (2) replicates per condition
- Shake flask cultures at 37°C, 5% CO2, 120rpm