Poster: Analysis Of Migration Using The Oris™ Cell Migration Assay-TriCoated Kit On The POLARstar Omega
The integrin family of proteins are essential for the ability of cells to adhere and to migrate on extracellular matrix components. There are two distinctive groups of integrins within the family that can be identified by their ability to bind to RGD containing extracellular matrix components such as fibronectin and those that act as collagen receptors. The migration of epithelial cell sheets can play an important role during development or wound healing. Phosphoinositide such as PtdIns3,4,5P3 and PtdIns4,5P2 are thought to play a role in migration. To study migration in cell lines, scratch assays were typically performed where a confluent monolayer was scratched to remove a strip of cells and then the movement of the cells from the edges into the gap was quantified. This method has several drawbacks including the removal of the ECM components and the rupturing of cells which could all skew the results.