Article | April 20, 2007
A Rapid Simultaneous Determination Of 33 Amino Acids And Dipeptides In Spent Cell Culture Media By Gas Chromatography-Flame Ionization Detection Following Solid And Liquid Phase Extraction
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•A Rapid Simultaneous Determination Of 33 Amino Acids And Dipeptides In Spent Cell Culture Media By Gas Chromatography-Flame Ionization Detection Following Solid And Liquid Phase Extraction
•A Rapid Simultaneous Determination Of 33 Amino Acids And Dipeptides In Spent Cell Culture Media By Gas Chromatography-Flame Ionization Detection Following Solid And Liquid Phase Extraction
•A Rapid Simultaneous Determination Of 33 Amino Acids And Dipeptides In Spent Cell Culture Media By Gas Chromatography-Flame Ionization Detection Following Solid And Liquid Phase Extraction
A rapid, sensitive and reproducible gas chromatographic method with flame ionization detection is described for the simultaneous identification and quantification of 33 amino acids (AA) and dipeptides in spent cell culture media in under 7 minutes. The method involves the use of the EZ:faastTM Amino Acid Analysis sample testing kit. Instrumental and assay precision, % recovery, linear range, limit of detection and peak identity in highly complex cell culture media containing either soy hydrolysate or Fetal Bovine Serum (FBS) were validated using a gas chromatography system with dual flame ionization detectors (GC-FID).
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•A Rapid Simultaneous Determination Of 33 Amino Acids And Dipeptides In Spent Cell Culture Media By Gas Chromatography-Flame Ionization Detection Following Solid And Liquid Phase Extraction
SOURCE: SAFC