Helpful Hints For Better Aseptic Technique By Bionique Testing Laboratories
Developing successful aseptic techniques requires specific training and a clear understanding of the nature, types, and potential sources
of contamination. Freshney (2005) and Ryan (2008a) provide good information on these critical areas. The following recommendations
are designed to help improve your aseptic techniques and to preserve the integrity of your cell cultures.
Article: Controls For Photometric Tests — Implications For Low Capacity Test Systems
Instrumentation for photometric endotoxin tests must have a capacity for at least 12 samples in order to meet the requirements of the
pharmacopeial endotoxins test chapters. Small test systems with a low capacity, including those that rely on archived curves, cannot
accommodate the controls specified in the pharmacopeia or guidance documents.
Application Note: The MIQE Guidelines And Assessment Of Nucleic Acids Prior To qPCR And RT-qPCR
Quantitative PCR has emerged as a fundamental technique in molecular and clinical diagnostics. Before now, little or no standardization in reporting existed, and as a result, experiments were frequently hard to replicate. In addition, many assumptions upon which the technique depends, in particular those pertaining to reference gene stability and amplification efficiency, were
often, in fact, invalid.