Application Note
Rapid and Efficient Determination of Kinetic Rate Constants
Surface plasmon resonance (SPR) optical biosensors are being used increasingly in a wide range of applications in basic biological research and pharmaceutical product development (Rich and Myszka 2005). We present here a significant advance in SPR biosensor technology: the ProteOn XPR36 protein interaction array system. The ProteOn XPR36 system incorporates a multichannel module and interaction array sensor chip for analysis of up to 36 independent protein interactions in a single injection step.
In a typical SPR biosensor experiment, a ligand is first immobilized onto a sensor chip surface and is then presented with an analyte in solution. The SPR biosensor detects the binding of the analyte to the ligand in real time and produces data on the association and dissociation kinetic rate constants of the reaction. When determining kinetic rate constants of a biomolecular interaction, a range of analyte concentrations is required to provide sufficient data for analysis. At the end of the binding step for a single analyte concentration, the ligand surface is normally regenerated before running the next analyte concentration.
In the ProteOn XPR36 protein interaction array system, there is no need to regenerate the ligand surfaces between samples because up to six analyte samples can be injected in parallel and in a single injection step. The capability of the ProteOn XPR36 protein interaction array system has been demonstrated for rapid and efficient determination of kinetic rate constants for protein interactions.
Source: Bio-Rad Laboratories, Inc.
Download The Complete Application Note
The content you requested is only available to registered users of Bioresearch Online. By registering now, you'll get exclusive access to this piece of content and thousands of addtional articles and product reviews across our entire network of sites.
