Application Note
Workflow For Protein Purification From Whey Using Nuvia™ Ion Exchange Media
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•Tech Note: Workflow For Protein Purification From Whey Using Nuvia™ Ion Exchange Media
By Bio-Rad Laboratories, Inc.
Whey proteins play a vital role in the formulation of food supplements because of numerous desirable nutritional and functional properties. Here we present a process for whey purification using Nuvia S and Nuvia Q ion exchange media. The major whey proteins are a- lactalbumin (ALA), ß- lactoglobulin (BLG), bovine serum albumin (BSA), and bovine immunoglobulins (Hahn et al.1998). Minor proteins such as lactoperoxidase, lactoferrin, and proteose-peptone account for the other protein components in whey.
Whey was obtained as a concentrate from Hilmar Ingredients. The solution was aliquoted, stored frozen at -20°c, and thawed before the experiments. The whey concentrate was diluted tenfold in equilibration buffer and filtered through a 1.2 micron filter (Sartorius Group) to remove particulates prior to chromatography.
Click Here To Download:•Tech Note: Workflow For Protein Purification From Whey Using Nuvia™ Ion Exchange Media
