Download
Poster: Volume Scaling Of The Transcreener® ADP2 FP Assay On The BioTek Synergy™ 2 And 4 Multi-Mode Microplate Readers Allows For Easy Transition From Assay Development To High-Throughput Screening
A critical factor in achieving success with an assay is to ensure that the assay detection instrumentation is compatible with the reagents being used. In addition to overall compatibility with the reader detection mode, assay reagents must also demonstrate scalability from larger volume to smaller volume wells to accommodate higher throughput processing.
We present data showing the validation of BellBrook Labs' Transcreener® ADP2 FP Assay on the BioTek Synergy™ 2 and Synergy™ 4 plate readers. The Transcreener ADP2 Assay is a universal, second generation far red fluorescence polarization assay that detects the ADP nucleotide by-product from any ATP-utilizing enzyme reaction. The Transcreener Assay has been validated in both 96- and 384-well plate formats on the Synergy 2 and 4 plate readers. Data generated with a standard curve in both microplate formats meet BellBrook Labs' Instrument Validation Program criteria, which include Z´ > 0.7 with ?mP shift = 95 at 10% conversion of 10 µM ATP to ADP. The 96-well assay met the validation criteria using the standard PMT. Although the 384-well assay performed well and achieved a Z´ = 0.6 using the standard PMT, the validation criteria were met using a red-shifted PMT.
The content you requested is only available to registered users of Bioresearch Online. By registering now, you'll get exclusive access to this piece of content and thousands of addtional articles and product reviews across our entire network of sites.
