Application Note
Using The NanoDrop 3300 Fluorospectrometer For Cancer Research Applications
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•Application Note: Using The NanoDrop 3300 Fluorospectrometer For Cancer Research Applications
By Thermo Scientific NanoDrop Products
Epigenetic modifications are defined as heritable information other than nucleotide sequences (1) that have the ability to control how and when transcription takes place. DNA methylation and secondary DNA structures such as DNA Gquadruplexes are among the epigenetic modifications currently under study because of their common associations with cancer (1,2). Fluorescence spectroscopy is being used to decipher these complex levels of cellular control at the molecular level, complimenting cell imaging and flow cytometry methods at the cellular level. In addition, fluorescence spectroscopy methodologies are aiding the targeting of and delivery to cancer cells in vivo to correct defects, inhibit growth, or kill cells (3).
The Thermo Scientific NanoDrop 3300 Fluorospectrometer uses a patented "cuvette-less" sample retention system for fluorescent measurements using as little as one microliter sample volume. With UV, blue and white LED excitation, the NanoDrop™ 3300 covers a broad wavelength range, thereby facilitating the use of many diverse fluorophores; it is also able to analyze multiple emission profiles from a single sample. The following examples describe some of the uses of NanoDrop™ micro-volume fluorescence technology as it pertains to cancer research.
Click Here To Download:•Application Note: Using The NanoDrop 3300 Fluorospectrometer For Cancer Research Applications
