Application Note
Use Of Custom Methods Capability Of NanoDrop Spectrophotometers For Toxicology Studies
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•Application Note: Use Of Custom Methods Capability Of NanoDrop Spectrophotometers For Toxicology Studies
By Thermo Scientific NanoDrop Products
Cell proliferation and cytotoxicity assays are commonly used to quantitatively analyze important cellular processes in order to assess disease states, toxicology, and drug efficacy and safety (1). Many cell proliferation/cytotoxicity assays rely on a tetrazolium dye which is reduced into a formazan product in the mitochondria of metabolically active cells. Spectrophotometric quantification of the formazan dye can be accomplished by measuring absorbance at 490 nm: decreased absorbance reflects lowered mitochondrial activity, and, by inference, decreased cell health. Many colorimetric cell proliferation/cytotoxcity assays are commonly analyzed using UV/Vis plate readers.
Thermo Scientific NanoDrop Spectrophotometers offer an attractive alternative for performing UV/Vis absorbance measurements. They utilize a patented sample retention technology that allows UV/Vis absorbance measurements to be carried out on volumes as small as 0.5 µl without the need for cuvettes or plates. This microvolume capability facilitates resampling of wells, multiplexing assays and significantly lowers setup and assay development costs. In addition, these instruments come with flexible, user-friendly software that includes pre-configured programs for common applications, as well as a custom method capability (Method Editor). This capability allows for development and sharing of methods tailored for user-specific needs.
Click Here To Download:•Application Note: Use Of Custom Methods Capability Of NanoDrop Spectrophotometers For Toxicology Studies
