Application Note

RSS

Click Here To Download:
•Technical Note: Virus Counter

By Applikon Biotechnology

The Virus Counter instrument and assay were designed to work with a variety of viruses from a range of sources. The non-specific virus staining procedure employed in the assay allows the same reagents to be used regardless of the virus to be analyzed. This document summarizes many of the viruses and samples that have been analyzed with the Virus Counter.

A two-stain mixture containing a nucleic acid-specific stain and a protein-specific stain is used for the sample preparation process; no antibodies are needed. These dyes are virtually non-fluorescent until they bind their targets so there is no need to remove excess dye in lengthy washing steps prior to analysis. Viral proteins (e.g., capsid proteins) are labeled with a fast, one-step stain that exhibits low protein-to-protein variability. The second stain in the dye mixture is also a fast, one-step stain with a high affinity for nucleic acids. This dye works quite well with all types of nucleic acids (i.e. single or double-stranded RNA or DNA). Because of this universal, non-specific sample preparation method almost any virus can be analyzed by the Virus Counter without having to supply your own sample-specific reagents.

Click Here To Download:
•Technical Note: Virus Counter