Application Note
Sartobind® Capsules For Capture Chromatography
Sartobind membrane adsorbers with 8 mm bed height (30 membrane layers) can be used for flow through and bind and elute (B&E) application as flow channels have been optimized for smallest void volume. Such optimization is essential to achieve sharp breakthrough curves, small elution volumes below two bed volumes (column volumes) and no back mixing of already separated solute samples.
The capsule design keeps the advantages of disposable Sartobind SingleSep® capsules with 4 mm bed height. They can be used for flow through applications due to the high flow rate, the clear scale and usage of same materials through the product line.
On the other hand the 8 mm capsules provide more membrane per capsule for higher binding capacity, they are reusable and meet the expectations for small elution volumes and peak resolution.
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