Application Note
Rapid Optimization Of Immobilization And Binding Conditions For Kinetic Analysis Of Protein-Protein Interactions Using The ProteOn XPR36 Protein Interation Array System
Click Here To Download:
•Application Note: Rapid Optimization Of Immobilization And Binding Conditions For Kinetic Analysis Of Protein-Protein Interactions Using The ProteOn XPR36 Protein Interation Array System
By Bio-Rad Laboratories, Inc.
The characterization of protein-protein interactions is a fundamental need in the rapidly growing field of proteomics. Surface plasmon resonance (SPR) technology is widely used for kinetic studies of protein-protein interactions. In the design of SPR experiments, however, determining the optimal conditions for ligand immobilization and analyte binding can often require some trial and error.
The ProteOn XPR36 protein interaction array system is an SPR biosensor with a multichannel module and interaction array sensor chip for analysis of up to 36 protein interactions in a single injection step. The parallel sample processing capability of the ProteOn XPR36 system can facilitate the determination of optimal reaction conditions. Because multiple conditions can be tested in parallel and in a single injection step, the effects of each condition on an interaction can be viewed in context of all the conditions simultaneously. Thus, the information needed to make progress toward a successful result is generated more quickly and with more flexibility than is possible with serial sample analysis. Additional details on this array-format system are provided in Bronner et al. (2006).
Click Here To Download:•Application Note: Rapid Optimization Of Immobilization And Binding Conditions For Kinetic Analysis Of Protein-Protein Interactions Using The ProteOn XPR36 Protein Interation Array System
