Application Note
Quality Control During Library Construction For de novo Next-Gen Sequencing Platforms
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•Application Note: Quality Control During Library Construction For de novo Next-Gen Sequencing Platforms
By Thermo Scientific NanoDrop Products
The arrival of Next Generation (Next-Gen) sequencing technologies has revolutionized the field of genomics by scaling up the sequencing process and allowing rapid acquisition of large amounts of data. The most common Next-Gen sequencing platforms currently available are (1) the Genome Analyzer systems by Illumina®, (2) the SOLiD™ systems by Applied Biosystems, and (3) the 454 Life Sciences™ systems by Roche. All three platforms require the construction of a randomly fragmented nucleic acid library with a highly specific fragment size range for de novo sequencing. There are a number of steps involved in library construction, and it is useful to monitor and track the process carefully to ensure that the majority of the fragments are the correct structure and the optimal size for the sequencing platform used. In most cases library preparation is an expensive process. Thus, it is prudent to include quality control steps throughout the library construction process to ensure the quality of downstream data.
The Thermo Scientific NanoDrop 2000c spectrophotometer is well suited for QC validation of Next-Gen sequencing library construction. Three features make the NanoDrop 2000c™ micro-volume spectrophotometer uniquely suitable for QC testing during library construction:
- Conservation of precious sample by requiring as little as 0.5 µl for measurement.
- Highly accurate concentration measurements.
- Purity assessment by determining the 260/280 and 260/230 ratios and displaying full spectral data for contaminant detection.
•Application Note: Quality Control During Library Construction For de novo Next-Gen Sequencing Platforms
