Profinity IMAC Media - Immobilized Metal Affinity Chromatography

Bio-Rad's Profinity IMAC Ni-charged and uncharged resins provide optimal purification of recombinant His-tagged proteins

Details

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•Datasheet: Profinity IMAC Resin
•Technical Note: Profinity IMAC Resin: An Optimal Support For His-Tagged Protein Purification
•Datasheet: Profinity IMAC Ni-Charged Resin
•Technical Note: Rapid Method Development With The BioLogic DuoFlow Chromatography System For The Purification Of His-Tagged Proteins

Bio-Rad's Profinity IMAC Ni-charged and uncharged resins provide optimal purification of recombinant His-tagged proteins.

Optimal ligand density for higher purity of target protein
Compatibility with denaturants, detergents, and reducing agents for purification over an expanded range of conditions
Stability in pH 1–14 for storage in a variety of solutions
Profinity polymer bead for purification at fast flow rates
Easy-to-pack resin for use in medium-pressure, gravity-flow, and spin columns
Available uncharged or precharged with Ni2

Profinity IMAC resins provide purification of recombinant His-tagged proteins over a wide molecular weight range. The Profinity IMAC bead is a porous 60 µm particle derivatized with iminodiacetic acid (IDA), which functions as the chelating ligand. The chemical structure of IDA, when charged with Ni2+or other transition metal ions, allows highly selective binding of recombinant His-tagged proteins over naturally occurring His-containing proteins. The polymeric nature, optimized IDA ligand density, and open pore structure of the Profinity IMAC bead result in superb mechanical strength, high selectivity for target proteins, low nonspecific binding, and the ability to perform purifications at faster flow rates.

Partial structure of Profinity IMAC resin. Illustration of UNOsphere bead with coupled IDA functional ligand, shown charged with Ni2+. Wavy lines indicate available binding sites.

Profinity IMAC resins, based on patented* UNOsphere technology, exhibit excellent flow properties without compromising binding, capacity, recovery, or purity.

* US patent 6,423,666.

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