Article
Performing Transductions In Cell Culture Vessels Which Allow For Screens Where High Cell Density Is Required
November 23, 2011
The high throughput analysis of potential drug candidates in a cellbased assay screen is, to say the least, complicated. New technologies are currently on the market to aid the assay biologist in developing and screening their drug candidates. Two such technologies include the BacMam Histone H3 [pSer10] Cellular Assay Kit (Life Technologies Cat. #A12898) and Corning's High Yield PERformance Flask (HYPERFlask) cell culture vessel. The baculovirus-mediated gene delivery system or BacMam in combination with the LanthaScreen® assay technology can be used for the analysis of specific posttranslational modifications of histones. The HYPERFlask vessel is a multilayer flask that uses a gas permeable film to provide efficient gas exchange between the cells, culture medium and the environment surrounding it. The experiments presented in this poster describe a protocol combining both technologies using HEK293 cells as the model. HEK293 cells containing the GFP-Histone H3 fusion gene were seeded into Corning white 384 well normal (Corning Cat.#3570) and low volume (Corning Cat.#3826) flat bottom microplates and assayed for LanthaScreen® activity using the PerkinElmer® Envision® plate reader containing Life Technologies supplied Terbium filters. The data show successful transduction of the HEK293 cells with the GFP-Histone H3 fusion gene utilizing both technologies and resulting in a high yield production of functional GFP-Histone H3 transduced cells.
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