Product/Service
PCR-TRAP Cloning System
GenHunter has developed a highly efficient blunt-end PCR-TRAP® Cloning System. This system uses a third generation cloning vector that features positive selection for cloning PCR products (see figures). Only recombinant plasmids confer antibiotic resistance, making PCR-TRAP® extremely efficient. There is no need for any post-PCR manipulation before cloning, since a significant fraction of PCR products do not contain the 3' overhanging A (Clark, 1988, Nucleic Acids Res. 16:9677).
The mechanism of this unique cloning system involves the phage Lambda repressor gene, cI, which has been incorporated into the PCR-TRAP® Vector. When transcribed, the gene codes for a repressor protein which binds to the Lambda right operators Or1 to Or3 of the cro gene, turning off the promoter which drives the TetR gene on the plasmid. Therefore, cloning the PCR product directly into the cI gene leads to the inactivation of the repressor gene and thus the expression of the TetR gene. So the PCR-TRAP® Cloning System is indeed a cloning TRAP!
The cloned PCR product can be quickly and easily retrieved by colony-PCR for the purpose of insert confirmation or probe generation using primers which flank the cloning site (provided in the kit). The flanking primers also allow the cloned PCR product to be readily sequenced.
See our website for a Partial Map of the PCR-TRAP® Vector and a list of references using the kit.
GenHunter Corp., 624 Grassmere Park Drive, Suite 17, Nashville, TN 37211. Tel: 615-833-0665; Fax: 615-832-9461.
