Product/Service
NFkB Antibodies
Product: anti-p65 (Rel A) [Rabbit]
Code: 100-4165
Lot # 4385
Size: 100 l
Physical State: Liquid (sterile filtered)
Antibody Concentration: 85.0 mg/ml (by Refractometry)
Buffer: None
Stabilizer: None
Preservative: 0.01% (w/v) Sodium Azide
Application(s): suitable for immunoprecipitation, immunoblotting, ELISA and supershift assays.
Recommended Dilution(s): This product was assayed by immunoblot and found to be reactive against NFkB p65 (Rel A) at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302. Anti-NFkB p65 (Rel A) is suitable for the detection by immunoblot of human and mouse NFkB p65 (Rel A). This product was also tested in a gel supershift assay and found to be reactive against all p65 (Rel A) containing human and mouse NFkB complexes using 0.5 to 1.0 l per assay. Optimal titers for other applications should be determined by the researcher.
Storage Conditions: Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is six (6) months from date of opening product.
Purity: This product was prepared from monospecific antiserum by delipidation and defibrination. Anti-NFkB p65 (Rel A) may react non-specifically with other proteins. Control peptide (code #100-4165p - provided with antiserum) will compete only with the specific reaction of antiserum with the NFkB p65 (Rel A) subunit.
Immunogen: NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Rockland Immunochemicals Inc., P.O. Box 316, Gilbertsville, PA 19525. Tel: 610-369-1008; Fax: 610-367-7825.
