Article
Improving The Accuracy & Speed Of Mammalian Cell Counting
October 18, 2007
•Article: Improving The Accuracy & Speed Of Mammalian Cell Counting
by Costas Ioannides
The skill of culturing mammalian cells successfully requires consistent, accurate measurements of cell growth and viability. Cell counts must be taken daily, a task that is not only tedious and time consuming, but also prone to error because of operator subjectivity. To that end, a simple device has been developed that uses well-documented fluorescence microscopy techniques to eliminate operator variances. The automated system detects fluorescent signals bound to cell nuclei, providing accurate cell counts in less than 60 sec. This report details the technology behind the NucleoCounter™ and its operation, including data from a run of Chinese hamster ovary (CHO) cells. Alternate counting methodologies, both manual and automated, are also discussed.
Reprinted from American Biotechnology Laboratory May 2003
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•Article: Improving The Accuracy & Speed Of Mammalian Cell Counting
SOURCE: Eppendorf, Inc
