Case Study
Filtration Trials With SAFC® EX-CELL® CD Hydrolysate Fusion
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•Case Study: Filtration Trials With SAFC® EX-CELL® CD Hydrolysate Fusion
Following the launch of the EX-CELL CD Hydrolysate Fusion, SAFC conducted a study with partner company Sartorius Stedim Biotech. EX-CELL CD Hydrolysate Fusion is the first fully chemically defined media supplement of its kind in the industry.
The purpose of the study was to determine the throughput and biological activity of each filter using the EX-CELL CD Hydrolysate Fusion mixture. SAFC provided the chemicals, media and solutions, while Sartorius Stedim Biotech provided all filters and related laboratory equipment. Cell line CHO-IgG, a proprie ztary chinese hamster ovary clone expressing a recombinant antibody, was used in conjunction with EX-CELL CD Hydrolysate Fusion powder that was mixed according to manufacturer's directions at 20x concentrate. Membrane materials were filtered using 0.2µm and 0.1µm sterilizing grade filters.
In order to measure the cell growth and recombinant protein production, the cells were placed in suspension shaker flasks and used to seed experiments that were conducted in 50 mL (30 mL working volume) TPP Bioreactor tubes. Initial cell density was 200,000 viable cells/mL. The cells were tested using three unique filters at a time in the Multitron Incubator.
Click Here To Download:•Case Study: Filtration Trials With SAFC® EX-CELL® CD Hydrolysate Fusion
