Application Note
Application Note: Enhancing Productivity Of CHO Cell Lines With DASGIP Parallel Bioreactor Systems
In industrial cell culture, Chinese Hamster Ovary (CHO) cells are the No. 1 work horse: They have been extensively studied and developed, and today provide a stable platform for producing monoclonal antibodies and recombinant proteins.
However, each (sub) clone may be different: growth characteristics, nutrition needs, and expression level can vary greatly between clones.
The sole dedication to maximize density can lead to unsuitable conclusions, as density does not necessarily mean higher productivity. One goal therefore can be to distinguish between the fast growers from the more productive variations among the sub clones of the same cell line. Another aspect of interest is the feeding strategy: Apart from glucose and glutamine feeds amino acids are critical to CHO growth and expression. In a nutshell: Enhancing productivity of CHO cell lines requires selecting the right sub-clone as well as the right cultivation parameter.
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