Application Note
E. coli Fermentation Using A Benchtop BioFlo 110 Fermentor
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•Application Note: E. coli Fermentation Using A Benchtop BioFlo 110 Fermentor
This Application Report is part of a series documenting culture growth in the BioFlo 110. With appropriate vessels and control modules, the BioFlo 110 can be used to efficiently grow mammalian cells, plant cells, insect cells, yeast, and bacteria.
For this report, a standard 7.5L BioFlo 110 Advanced Fermentation Kit, NBS Catalog Number M1273-1125 was first used for an E. coli fed-batch fermentation.
Next, a BioFlo 110 Gas-Mix Controller, M1273-3104, was added, and the fermentation repeated with oxygen supplementation of the sparge gas. This second culture, described in the APPENDIX, achieved a very high dry cell weight of 57.2 g/L. Neither run was fully optimized, but the descriptions of procedures and materials as well as the data discussion will be useful to operators of similar fermentors.
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•Application Note: E. coli Fermentation Using A Benchtop BioFlo 110 Fermentor
SOURCE: Eppendorf, Inc
