Application Note
Tech Note: Culturing V79-4 Cell Line On A Thermo Scientific Nunc Nunclon Cell Culture Treated Surface
Thermo Scientific Nunc Nunclon cell culture products are tested for cell growth and plating efficiency using several different cell lines.
Nunc™ Nunclon™ products are tested with two cell lines L929, HEL 299 or F2002 and one Primary Chick Embryo cell culture for monolayer formation, plus cell line V79-4 for cloning efficiency.
V79-4 is a fibroblast-like cell line derived from the lung tissue of a male Chinese hamster. It has a relatively high plating efficiency and short generation time.
This Tech Note describes a procedure for culturing V79-4 cell line on a Nunclon treated surface.
Materials and Methods
- V79-4 cells (ATCC CCL 93)
- Minimum Essential Medium Eagle (MEM)
- Bovine Calf Serum (BCS), iron supplemented
- Fetal Bovine Serum (FBS)
- L-Glutamine, 200 mm
- Non-essential Amino Acids (NEAA), 100X
- Dulbecco's Phosphate Buffered Saline, 1X (without Ca2+ or Mg2+)
- Trypsin Solution, 1X
- Antibiotic/Antimycotic Solution, 100X
- Crystal Violet or Methyl Violet, 0.1-0.4% in aqueous alcohol solution
- Product controls
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