Product/Service

RSS

• The only clone truly derived from the avian myeloblastosis virus
  
• Maintains the RNA- and DNA-dependent DNA polymerase and RNase H activities
  
• RNase H activity can be regulated over a wide range of temperatures
  
• Expressed as a very stable, highly active polymer
  
• Remarkably robust in cDNA synthesis and RT-PCR
  
• Capable of synthesizing cDNA over a wide range of temperatures
  
• Ideal for use in RT-PCR, cDNA libraries, and dideoxy-DNA sequencing
  

Storage Buffer:

200 mM potassium phosphate, pH 7.2, 2.0 mM (v/v) Triton X-100 and 50% (v/v) glycerol.

Assay Conditions:

50 mM Tris-HCl, pH 8.3, 6.0 mM MgCl2, 1.0 mM, 40 mM KCl, 0.5 mM [3H]dTTP, 0.2 mM poly(rA)·(dT)50 in a reaction volume of 50 µl.

Quality Control:

All preparations are assayed for contaminating endonuclease and exonuclease and nonspecific RNase and single- and double- stranded DNase activities.

References:

1. Goodman, H.M., and MacDonald, R.J. (1979) Methods Enzymol. 68, 75-90 2. Naylor, L.H., and van de Sande, J.H. (1986) Nucleic Acids Res. 14, 5939 3. Zagursky, R.J., Baumeister, K., Lomax, N., and Berman, M.L. (1985) Gene Anal. Techn. 2, 89-94 4. Houts, G.E., Masakau, M., Ellis, C., Beard, D., and Beard, J.W. (1979) J. Virol. 29, 517-522

CHIMERx, 5520 W. Burleigh St., Milwaukee, WI 53210. Tel: 414-535-8585; Fax: 414-535-9508.