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BioSepra Blue Trisacryl® M is an affinity chromatographic sorbent used for the purification of a wide variety of enzymes and proteins such as kinases, albumin, interferons, and some coagulation factors.

The basic matrix is Trisacryl GF2000, a macroporous non ionic sorbent on which Cibacron Blue is covalently immobilized. The initial matrix is a copolymer of N-acryloyl-2-amino-2 hydroxymethyl-1,3-propane-diol in bead form. Its macroporous structure allows a good diffusion and improves exchange kinetics.

Cibacron blue F3GA dye is strongly bound to the matrix through a six carbon spacer arm (Figure 1). The reaction is performed with the EEDQ coupling agent. This type of coupling prevents any leakage of the dye in normal working conditions.

Chemical and Mechanical Stability

The excellent chemical stability of Blue Trisacryl M is a function of the synthetic nature of Trisacryl sorbent and the enhanced stability of the ligand coupling mechanism. The sorbent has an excellent mechanical stability. High flow rates with working pressures up to 3 bar can be used.

Experimental results have shown that the flow rate increases linearly with pressure up to about 40 cm per hour. However, the absolute linear flow rate and its relation to working pressure will vary according to the column height. Due to their extended lifetime, Blue Trisacryl M columns do not require frequent repacking, which is cost and labor-saving.

Experimental results showed no modification of the chromatographic properties of the sorbent after more than 100 cycles of albumin separation from whole plasma. On an industrial scale, Blue Trisacryl M was used over 700 cycles in the production of human albumin from IgG depleted plasma.

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