Product/Service
Bio-Scale Mini Cartridges - Prepacked Chromatography Columns
•Datasheet: Bio-Scale™ Mini Cartridges
Bio-Scale Mini cartridges have a patent-pending doublewall cartridge design that provides extra durability and allows easy, reliable runs at pressures up to 45 psi with the aqueous buffers most commonly used for protein separation. The cartridges are prepacked with Bio-Rad's chromatography media for ion exchange, affinity, and size exclusion. The cartridges come in 1 and 5 ml formats that can be used for method scouting or simple purifications. Larger 10 and 50 ml desalting cartridges are also available. Bio-Scale Mini cartridges are convenient, disposable, and ready to use.
The cartridges are designed for use with BioLogic systems, a peristaltic pump, or any chromatography system. Bio-Scale Mini affinity cartridges can also be run on the Profinia protein purification system. For simple step elution, the cartridges can be used with a Luer-Lok syringe. Fittings are available for connection to HPLC- and FPLC-type systems. Other features include:
- Simple luer fittings for easy connection to any system
- Chemically compatible polypropylene parts
- A wide range of chromatography media
| Prepacked
Cartridge Selection by Application |
||||
| Cartridge
by Application |
Support |
Type
of Separation |
Chemical
Form |
Protein
Capacity/ml* |
| Protein
and Plasmid Purification |
||||
| CHT
Ceramic Hydroxyapatite |
Ceramic
Hydroxyapaptite |
Affinity Ion Exchange |
Ca2-
, |
Type
1: >25 mg/lysozyme/g Type II: >12.5 mg lysozyme/g |
| High
Q |
Macro-Prep
High Q |
Strong
anion exchange |
–N+(CH3)3 |
>37
mg BSA |
| UNOsphere
Q |
UNOsphere
Q |
Strong
anion exchange |
–N+(CH3)3 |
180
mg BSA |
| UNOsphere
S |
UNOsphere
S |
Strong
cation exchange |
–SO3- |
60
mg human lgG |
| DEAE |
Macro-Prep
DEAE |
Weak
anion exchange |
–HN+(C2H5)2 |
>35
mg protein |
| Protein
Purification |
||||
| CHT
Ceramic Hydroxyapatite |
Ceramic
Hydroxyapaptite |
Affinity Ion Exchange |
Ca2-
, |
Type
1: >25 mg/lysozyme/g Type II: >12.5 mg lysozyme/g |
| High
S |
Macro-Prep
High S |
Strong
cation exchange |
–SO3- |
>49
mg human lgG |
| lgG
Purification |
||||
| DEAE
Blue |
DEAE
Affi-Gel Blue |
Affinity |
Cibacron
Blue F3GA and DEAE |
0.2–1.0
ml serum |
| Antibody
Purification |
||||
| CHT
Ceramic Hydroxyapatite |
Ceramic
Hydroxyapaptite |
Affinity Ion Exchange |
Ca2-
, |
Type
1: >25 mg/lysozyme/g Type II: >12.5 mg lysozyme/g |
| Protein
A |
Affi-Prep
protein A |
Affinity |
Protein
A |
8–10
mg mouse monoclonal lgG1 16–23 mg human lgG |
| Affinity-Tagged
Protein Purification |
||||
| IMAC |
Profinity
IMAC |
Affinity |
Iminodiacetic
acid |
>15
mg recombinant His-tagged protein |
| GST |
Profinity
GST |
Affinity |
Glutathione |
>7
mg/ml recombinant GST-tagged protein |
| eXact |
Profinity
eXact |
Affinity |
Modified
subtilisin protease |
>3
mg tag-free MBP/ml resin |
| Desalting,
Buffer Exchange |
||||
| P-6 |
Bio-Gel
P-6 |
Gel
filtration |
Polyacrylamide |
10–22%
CV** |
| Purification
of Serum Proteins, Enzymes |
||||
| Blue |
Affi-Gel
Blue |
Affinity |
Cibacron
Blue F3GA |
0.2
ml serum 11 mg albumin |
| *
Purification capacity based on bulk support; check individual instruction
manuals for run conditions and specifications. ** Column volume. |
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•Datasheet: Bio-Scale™ Mini Cartridges
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