Application Note
Applications Of The ProteOn NLC Sensor Chip: Antibody-Antigen, DNA Protein, And Protein-Protein Interactions
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•Technical Note: Applications Of The ProteOn NLC Sensor Chip: Antibody-Antigen, DNA Protein, And Protein-Protein Interactions
By Bio-Rad Laboratories, Inc.
The ProteOn NLC sensor chip is one of several types of sensor chips designed for use with the ProteOn XPR36 protein interaction array system. The chip is coated with a layer of immobilized NeutrAvidin used for single-step immobilization of various biotinylated biomolecules (for example, peptides, proteins, oligonucleotides, and carbohydrates). The neutral charge of this NeutrAvidin layer minimizes nonspecific binding, and also enhances immobilization of oligonucleotides because their negative charge does not interfere with surface immobilization. Terminal labeling of immobilized oligonucleotides with biotin ensures high accessibility to analyte molecules, and no surface activation or deactivation steps are required, making the NLC immobilization protocol both rapid and effective.
Click Here To Download:•Technical Note: Applications Of The ProteOn NLC Sensor Chip: Antibody-Antigen, DNA Protein, And Protein-Protein Interactions
