Product/Service

Alkaline Phosphatase (E. coli)

Source: CHIMERx
One unit is the amount of enzyme required to hydrolyze 1 µmol of p-nitrophenylphosphate in 1 min at 37°C in a buffer of 1.0 M diethanolamine, 10 mM p-nitrophenylphosphate, 0.25 mM MgC12, pH 9.8.
Details
  • Catalyzes the hydrolysis of phosphate esters, including those present in nucleic acids and nucleotides
  • More thermal stable than Calf Intestine Alkaline Phosphatase (CIAP)
  • Optimal incubation temperature is approximately 60°C, however the enzyme remains active from 20°C to 80°C
  • Resistant to chemical changes and active over a broad range of buffer conditions
  • Can be used to remove 5'-phosphates from DNA or RNA prior to 5'- labeling
  • Works to remove 5'-phosphates from linearized vector molecules to prevent self-ligation of the vector during cloning procedures (1)
  • Ideal for diagnostic immunoassays and immunodetection of proteins and nucleic acids following blotting experiments
Storage Buffer:
20 mM Tris-HC1, pH 7.0, 5 mM KPO4, 100mM KC1, 0.1 mM MgC12, 0.1 mM ZnC12 plus stabilizers.
Quality Control:
All preparations are assayed for contaminating endonuclease and nonspecific RNase and single- and double-stranded DNase activities.
References:
1. Maniatis, T., Fritsch, E.F., and Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual, pp. 133-134, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

CHIMERx, 5520 W. Burleigh St., Milwaukee, WI 53210. Tel: 414-535-8585; Fax: 414-535-9508.

Most Popular

more...
Need Information?

Please wait... busy