Product/Service


Agarase

Source: CHIMERx
One unit is the amount of enzyme required to completely degrade 200 µl of molten 1% agarose in reaction buffer in 1 hour at 41°C. After digestion, agarose will not solidify when incubated at 4°C for 1 hour.
Details

  • Potent enzyme that digests the polysaccharide backbone of agarose yielding ethanol soluble oligosaccharides
  • Resulting carbohydrate molecules no longer gel or interfere with subsequent DNA manipulations
  • Ideal for quantitative recovery of high molecular weight DNA from low-melting agarose gels
  • Simple, quantitative recovery of intact nucleic acids from agarose gels
  • Can be heat inactivated (2 minutes at 95°C or 15 minutes at 65°C)
  • May be used to purify large (>50 Kb) and small (<50 Kb) DNA fragments from agarose gels
  • DNase and RNase free

Reaction Buffer:
40 mM Bis-Tris, pH 6.0, 40 mM NaCl, 0.5 mM EDTA.

Quality Control:
All preparations are assayed for contaminating endonuclease and nonspecific RNase and single- and double-stranded DNase activities. Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis.

CHIMERx, 5520 W. Burleigh St., Milwaukee, WI 53210. Tel: 414-535-8585; Fax: 414-535-9508.

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