Product/Service
Single-Step Affinity Purification And Fusion-Tag Cleavage Consumables
•Datasheet: Profinity eXact Cloning and Expression Kits
•Brochure: Profinity eXact Fusion-Tag System
•Datasheet: Profinity eXact Resin
•Technical Note: Profinity eXact Fusion-Tag System Performs On-Column Cleavage And Yields Pure Native Protein From Lysate In Less Than An Hour
Profinity eXact purification resin
The Profinity eXact purification resin consists of a highly engineered subtilisin protease conjugated to an agarose based matrix. Resulting purity of the eluted protein is typically higher than that for other affinity-tag systems due to the specific recognition of subtilisin for its prodomain sequence. The resin can be packed into different column formats, including low- to medium-pressure columns, gravity-flow columns, and mini spin columns, offering users added purification flexibility. Additional advantages of Profinity eXact purification resins include:
- Purification and processing of fusion-tagged proteins in a single-step
- On-column cleavage in as little as 30 minutes
- No protease addition required
- Precise cleavage at the N-terminus of the target protein to generate native protein sequence*
Resin is supplied in 100 mM sodium phosphate, pH 7.2 containing 0.02% sodium azide. It is also available prepacked in 1 ml and 5 ml cartridges, and in mini spin columns.
Profinity eXact expression and purification starter kit
The Profinity eXact expression and purification starter kit can be used to easily evaluate Profinity eXact fusion-tag technology and is suitable for new purifications requiring tag removal, and for purifications where cleavage has resulted in inferior results. The Profinity eXact expression and purification starter kit includes two kits: the Profinity eXact cloning and expression starter kit for the cloning and expression of a target gene using pPAL vectors and competent cells, and the Profinity eXact mini spin purification starter kit for single-step purification and on-column cleavage of the tagged protein. The mini spin purification starter kit also includes a lyophilized lysate to ensure that preliminary purifications are a success.
Click Here To Download:
•Datasheet: Profinity eXact Cloning and Expression Kits
•Brochure: Profinity eXact Fusion-Tag System
•Datasheet: Profinity eXact Resin
•Technical Note: Profinity eXact Fusion-Tag System Performs On-Column Cleavage And Yields Pure Native Protein From Lysate In Less Than An Hour
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