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•Application Note: Versatile, Cost-Effective Automation Of Avian Influenza
Mycoplasma Gallispeticum-Synovaie ELISAs

By Wendy Goodrich, Applications Scientist, BioTek Instruments, Inc.
Matt Durgin, PAS Technical Service Rep, IDEXX Laboratories

Designing effective automated assay workflows is a challenge for many laboratories. There can be a number of obstacles to achieving favorable throughputs. For example, when optimizing reagent volumes used by instrumentation, it is important to conserve reagents, especially target specific ones that can be costly to manufacture, and may not be offered for individual purchase. This presents a significant workflow problem as adding reagent overages or ‘dead' volumes required by the automation to the actual volume required to process the assay may total a volume greater than the minimum required reagent provided in the kit.

Strictly defined incubation steps that include specified windows of time and temperature are a typical specification for ELISA kits. Variable incubation times that fall outside of the specification can be a significant factor contributing to reduced assay performance within and between microplates. The shortest incubation time defined by the kit will generally limit the total number of plates that can be effectively processed in a batch without introducing the possibility of assay signal drift from plate to plate. Seamlessly integrating defined incubation windows into a workflow avoids these potential problems.

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•Application Note: Versatile, Cost-Effective Automation Of Avian Influenza And Mycoplasma Gallispeticum-Synovaie ELISAs