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Use Of CyDye Fluors For Improved FRET Protease Assays On BMG POLARstar Fluorescence Microplate Reader
October 5, 2006
•Application Note: Use Of CyDye Fluors For Improved FRET Protease Assays On BMG POLARstar Fluorescence Microplate Reader
Members of the matrix metalloproteinase (MMP) family play an important role in tissue remodeling and repair. However, inappropriate MMP activity has been implicated in a number of disease states including arthritis, tumour invasion and metastasis, and cardiovascular disease. The development of agents to control MMP activity continues to be a major focus for the pharmaceutical industry.
Many synthetic MMP peptide substrates have been described which incorporate a fluorophore and quencher moiety positioned either side of the enzyme cleavage site.1-3 We have prepared a number of CyDye labelled MMP substrates. Cleavage of these with specific MMP enzymes produces improved and signifi cant fluorescence increase at donor fluorophore wavelengths when compared to traditionally used fluors.
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•Application Note: Use Of CyDye Fluors For Improved FRET Protease Assays On BMG POLARstar Fluorescence Microplate Reader
SOURCE: BMG LABTECH, GmbH
