Articles
Use Of Chemically Defined Supplements In Optimizing The Performance Of CHO Cells
August 5, 2009
•Poster: Use Of Chemically Defined Supplements In Optimizing The Performance Of CHO Cells
By Ashley D. Smith, Avril A. Lawshé, Dennis Cooley, Matthew V. Caple, and James S. Ross
Chinese Hamster Ovary (CHO) cells are widely used by the biopharmaceutical industry for the production of therapeutic recombinant proteins. The enhancement of cellular productivity can be achieved through the use of many techniques, including the delivery of nutritional supplements (feeds) during cell culture. Historically, undefined supplements, such as protein hydrolysates, have been used to increase both the cell growth and productivity in fed-batch processes. However, both regulatory concerns and variability associated with undefi ned components have discouraged the use of such additives, creating a need for chemically defined (CD) feeds. In response to this need, a platform of CD supplements has been developed using spent media analysis and historical data to understand the nutritional and physiological requirements of CHO cells in suspension culture.
In the present study, we have examined the effect of undefined and CD supplementation on the growth and productivity of multiple CHO cell lines cultured in various chemically defi ned media. We observed a signifi cant increase in growth and productivity when cells were supplemented with feeds from the CD platform as compared to a glucose-only feed. This increase was comparable to the effect seen when cultures were supplemented with undefined protein hydrolysates, though the degree to which growth and productivity increases is dependent on cell line and media formulation.
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