Articles
Rapid Ultra-Sensitive Isothermal DNA Detection Using RPA Technology And The POLARstar OPTIMA
March 30, 2009
•Application Note: Rapid Ultra-Sensitive Isothermal DNA Detection Using RPA Technology And The POLARstar OPTIMA
By Olaf Piepenburg and Niall A. Armes TwistDX Ltd.
Nucleic acid amplifi cation techniques (NAATs) are central to molecular DNA tests, including clinical diagnostics, environmental testing, food testing, and many other applications. While PCR-based testing in laboratories is well established, thermo-cycling equipment that can monitor reaction kinetics is expensive, as well as power-demanding and generally unsuitable in providing cost-effective access to a large number of end-users.
Expansion of the user market requires reduction in the cost complexity and power requirement of devices and a simplifi - cation of operating procedures. The isothermal nucleic acid amplifi cation technology Recombinase-Polymerase-Amplification (RPA), recently developed by TwistDx, is ideally positioned to fulfil these requirements. The constant low reaction temperature of RPA, its resistance to temperature fluctuation, and the integration of the DNA amplifi cation step with proprietary detection probes means that it can be employed easily using standard laboratory equipment such as the POLARstar OPTIMA microplate reader from BMG LABTECH as well as small portable devices anticipated in the near future.
Probes for the RPA system use a fluorophore and quencher separated by a nuclease target site which operates only on duplex DNA – thus fluorescence is seen to increase when specific amplification has occurred.
Click Here To Download:•Application Note: Rapid Ultra-Sensitive Isothermal DNA Detection Using RPA Technology And The POLARstar OPTIMA
