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•Application Note: Predictor™ hERG Fluorescence Polarization Assay Kit Performed On The PHERAstar/PHERAstar Plus

By E.J. Dell, BMG LABTECH, Durham, NC USA

To address this need, the Predictor™ hERG Fluorescence Polarization Assay Kit from Invitrogen has been developed2. The assay is based upon the principle of fluorescence polarization (FP), in which a red-shifted fluorescent tracer is displaced from the hERG channel by compounds that bind to the channel. When the tracer is bound to the channel the FP value is high, and when displaced by hERG binding compounds the FP value is low.

The assay kit includes a stable membrane preparation that contains high levels of hERG protein, as well as optimized tracer, buffer, and controls necessary to perform the assay. This technical note describes the performance of the PredictorTM hERG Assay on the BMG LABTECH's PHERAstar/PHERAstar Plus multifunctional HTS microplate reader.

BMG LABTECH's PHERAstar/PHERAstar Plus is a multidetection microplate reader that combines rapid plate reading necessary for high throughput screening (HTS) with enhanced performance and sensitivity needed to read small fluid volumes. The PHERAstar/ PHERAstar Plus has been designed to read all HTS detection modes (fluorescence intensity, time-resolved fluorescence, fluorescence polarization, luminescence, and absorption) in all plate formats up to 1536 wells. The PHERAstar/PHERAstar Plus uses a unique application-specific optic module in conjunction with a reading head featuring five photomultiplier tubes that can simultaneously measure two emission signals at any desired wavelength. This Simultaneous Dual Emission (SDE) detection on the PHERAstar/ PHERAstar Plus provides for outstanding sensitivity and accuracy in fluorescence polarization assays, minimizing read times and increasing Z' values.

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•Application Note: Predictor™ hERG Fluorescence Polarization Assay Kit Performed On The PHERAstar/PHERAstar Plus