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•Optimizing Medium For Specific Chinese Hamster Ovary (CHO) Clone Cells

Chinese Hamster Ovary (CHO) cells are used extensively to produce recombinant proteins that require post-translation modifi cation to express full biological function. Recombinant CHO cells are challenging to culture because each clone has diverse nutritional requirements. To save critical development time, we have developed very effi cient medium optimization processes.

When optimizing medium for a specific CHO clone, pharmaceutical companies frequently follow a traditional approach of testing one medium component at a time to determine its optimal level. This strategy is labor intensive, costly and time consuming. To reduce costs and decrease development time, we adopted a newer approach based on statistical design. Design of Experiment (DOE) software can be customized to a user's needs and desired development criteria. We can analyze multiple criteria simultaneously to determine synergistic responses between them. We can then identify one or more media mixtures that provide the desired user outcomes.

SAFC Biosciences (SAFCB)' CHO cell culture platform, that includes high-throughput screening technology, a diverse selection of CHO media (both animal-component free and chemically-defi ned formulae), state-of-the art cell engineering, and DOE design software, allows us to make CHO medium optimization an extremely effi cient procedure. We will present the new strategic approach by making "CHO Media Library" (Figure 1) to speed medium development. An example depicts use of the "CHO Media Library" combining DOE analysis to effi ciently develop several optimized formulations for a humanized IgG-producing CHO clone.

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•Optimizing Medium For Specific Chinese Hamster Ovary (CHO) Clone Cells

SOURCE: SAFC