Articles
Nutrient Optimization For Mammalian Cell Culture
February 26, 2007
•Nutrient Optimization For Mammalian Cell Culture
Large-scale mammalian cell-culture–based manufacturing is complex and has many facets including cell line development, media and feed optimization, bioreactor process design, and downstream protein retrieval and purification. Batch and fed-batch modes of culture are frequently used because they offer ease of operation, scalability, good process control, and concentrated end-product. Batch cultures use media that supply all nutrients at the start of the run, whereas fed-batch cultures are initiated in a basal formulation and supplemented with interval feeds. Feeding cultures helps maintain the nutritional environment for cells during the course of the culture and helps limit the production of metabolic byproducts. The resulting advantages are seen in increased maximum viable cell density and extended culture duration. This leads to a cumulative increase in productivity and increased manufacturing efficiency in comparison to batch culture.
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•Nutrient Optimization For Mammalian Cell Culture
SOURCE: SAFC
