Articles
Molecular Probes PicoGreen® Assay Performed On BMG LABTECH POLARstar OPTIMA Microplate Reader
November 1, 2006
•Application Note: Molecular Probes PicoGreen® Assay Performed On BMG LABTECH POLARstar OPTIMA Microplate Reader
State of the art biotechnology relies on many different analytical techniques in combination with high throughput screening (HTS) methodology. The detection and quantitation of minute amounts of DNA is routinely done in a high percentage of these applications.
One of the most common methods for nucleic acid detection is the measurement of solution absorbance at 260 nm (A260). Although a relatively simple method, A260 suffers from low sensitivity and interference from nucleotides and single-stranded nucleic acids.
Furthermore, compounds commonly used in the preparation of nucleic acids absorb at 260 nm leading to abnormally high quantitation levels.1 New fl uorescent staining techniques can be much more sensitive and specifi c for double stranded DNA (dsDNA) than absorbance methods. The PicoGreen® dsDNA quantitation reagent from Molecular Probes is a highly sensitive fl uorescence assay for dsDNA detection.
PicoGreen® is widely used in industry and is quickly becoming a standard dsDNA quantitation method due to the high degree of sensitivity, linearity, and dynamic range achievable.
This application note investigates a method for using the PicoGreen® assay on the BMG LABTECH POLARstar OPTIMA microplate reader.
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•Application Note: Molecular Probes PicoGreen® Assay Performed On BMG LABTECH POLARstar OPTIMA Microplate Reader
SOURCE: BMG LABTECH, GmbH
