Articles
Integrated Strategies For Clone And Media Formulation Selection
March 26, 2010
• Article: Integrated Strategies For Clone And Media Formulation Selection
By Mark A. Gerber, Alahari Arunakumari, Dan Huang, Joel Goldstein, Sandra McNorton, Barry Drew, Matthew V. Caple, Jeanette M. Hartshorn, Michael Wathen, Kevin J. Kayser
Clone selection techniques used for development of stable, high-expressing recombinant cell lines suitable for robust fed-batch cell culture processes are critical for biopharmaceutical manufacturing. Basal media screening, feed development and addition strategies, and fed-batch bioreactor performance are all intimately tied to overall performance of the clones during scale-up. Serious issues can arise if a high-quality clone is not established, such as low or unstable protein yield and ineffective use of costly resources.
Reprinted with permission from BioProcess International
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