Articles

RSS

Click Here To Download:
•Feature Article: Improved Expression Vector Activity Using Insulators And Scaffold/Matrix-Attachment Regions

By by Helen Y. Kim

With recombinant proteins continuing to emerge as an important class of human therapeutics, improved methods to generate cell lines expressing high levels of desired proteins are becoming increasingly critical to the industry. Highexpressing cell lines are important for production of clinical candidate molecules as well as in rapid and reliable production for characterization and validation studies. In either case, the method should be rapid, costeffective, and scalable.

Specifically, a method that generates high-expressing cell clones without an extensive selection and screening step would be useful for production of clinical candidates. A method to rapidly and reliably generate proteins in hundreds of milligrams would be equally valuable for early phase validation studies. For such production, using a pool or a collection of transfected cells rather than a single cell clone can significantly reduce necessary resources and time. But widely variable expression levels in different cell clones from a transfection necessitates extensive single-cell cloning and screening, which prevents the use of transfected cell pools for even small-scale production. Furthermore, expression levels typically decrease with culture time.

Reprinted with permission from BioProcess International 4(5):S24-S31 (May 2006)

Click Here To Download:
•Feature Article: Improved Expression Vector Activity Using Insulators And Scaffold/Matrix-Attachment Regions