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Homogeneity Analysis And Cell Culture Performance Of Powder Cell Culture Media Manufactured With A Novel Continuous Flow Milling Process
February 26, 2007
•Homogeneity Analysis And Cell Culture Performance Of Powder Cell Culture Media Manufactured With A Novel Continuous Flow Milling Process
A novel, high-throughput continuous milling process for the manufacture of dry powder cell culture media has been designed, consisting of a pin mill combined with three-dimensional conical mixer technology. The purpose of this study was twofold: first, to demonstrate the homogeneity of key components in powder cell culture media manufactured using the continuous mill; and second, to verify that media manufactured in the continuous mill performs consistently and in a manner equivalent to ball-milled media when used in cell culture. A 2400 kg lot of basal media and an 800 kg lot of serum-free media were formulated, milled, blended and packaged using the continuous milling process. Powder media samples were obtained from various locations and depths within the postmill blender and final packaging containers and analyzed for amino acids, vitamins and trace metals. The analyses confirm that the milling and blending parameters established for milling process produce homogeneous media formulations. Cell culture studies were performed and indicate that media produced using the continuous milling process performed consistently throughout the batch and in a comparable manner to ball-milled media.
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•Homogeneity Analysis And Cell Culture Performance Of Powder Cell Culture Media Manufactured With A Novel Continuous Flow Milling Process
SOURCE: SAFC
