Articles
Fluorescence Polarization Discriminates Green Fluorescent Protein From Interfering Autofluorescence
October 5, 2006
•Application Note: Fluorescence Polarization Discriminates Green Fluorescent Protein From Interfering Autofluorescence
Green fluorescent protein (GFP) has in recent years been widely adopted as a versatile marker for reporting on gene expression in a wide range of cells and organisms. However, unless very highly expressed, fluorescence measurements of GFP are invariably contaminated with endogenous autofluorescence from the cells or media. This is often significant at the same wavelengths as GFP, making discrimination by simple modifications to the filter set relatively ineffective. This paper describes a novel and patent protected use of the fluorescence polarization (FP) optics available on the POLARstar from BMG LABTECH, which can dramatically increase the resolution of GFP fluorescence in the presence of unwanted autofluorescence. The POLARstar has been used to distinguish the expression of GFP in yeast cells in an assay for genotoxicity, where the test compounds were themselves highly autofluorescent and would ordinarily mask GFP, making toxicity assessment impossible.
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•Application Note: Fluorescence Polarization Discriminates Green Fluorescent Protein From Interfering Autofluorescence
SOURCE: BMG LABTECH, GmbH
