Articles

RSS

Click Here To Download:
•Application Note: Fluorescence Analysis Of Reactive Oxygen Species (ROS) Generated By Six Isolates Of Aspergillus Fumigatus

Reactive oxygen species (ROS) are essential intermediates in oxidative metabolism. Nonetheless, when generated in excess, ROS can damage cells by peroxidizing lipids and disrupting structural proteins, enzymes and nucleic acids. Excess ROS are generated during a variety of cell stresses, including ischemia/reperfusion, exposure to ionizing and ultraviolet radiation and/or inflammation. Reactive oxygen species (ROS) may contribute to inflammation and tissue damage.

The generation processes of reactive oxygen species can be monitored using the luminescence ana-lysis or also fluorescence methods. The intracellular ROS generation of cells can be investigated using the 2',7'-dichlorfluorescein-diacetate (DCFH-DA) as a well-established compound to detect and quantify intracellular produced H2O2.1 The conversion of the nonfluorescent 2',7' - dichlorfluorescein - diacetate (DCFH-DA) to the highly fluoresecent compound 2',7'-dichlorfluorescein (DCF) happens in several steps. First, DCFH-DA is transported across the cell membrane and deacetylated by esterases to form the non-fluorescent 2',7'-dichlorfluorescein (DCFH). This compound is trapped inside of the cells. Next, DCFH is converted to DCF through the action of peroxid rated by the presence of peroxidase.²

Aspergillus species are of further interest in the pathogenesis of several dermatological diseases. It is uncertain whether Aspergillus itself may generate ROS and therefore actively induce tissue damage. The present study investigates whether Aspergillus species are capable to produce ROS by themselves and if there are differences between the several strains.

Click Here To Download:
•Application Note: Fluorescence Analysis Of Reactive Oxygen Species (ROS) Generated By Six Isolates Of Aspergillus Fumigatus

SOURCE: BMG LABTECH, GmbH