Articles
Development Of Animal Component-Free Medium For Virus Production
February 26, 2007
Click Here To Download:•Development Of Animal Component-Free Medium For Virus Production
The use of wild-type reovirus has been developed as a cancer treatment by Oncolytics Biotech (Reolysin™). We developed an animal component-free cell culture medium to facilitate the production of this virus as a pharmaceutical agent. The development was initiated with an intensive media screen. From this screening phase, we were able to identify not only a formulation for further optimization but also some critical components for virus productivity. More over, in the optimized formulation SAFC Biosciences Item No. 65719, the cells generated less "metabolic waste" ammonia and utilized "metabolic wastes" glutamate and lactate when the energy sources such as glutamine and glucose were depleted. Formulation Item No. 65719 supported viable cell densities near 9E6cells/mL and Tissue Culture Infecting Dose (TCID50/mL) value greater than 5E10/mL were achieved after medium and process optimization.
Click Here To Download:
•Development Of Animal Component-Free Medium For Virus Production
SOURCE: SAFC
