Articles
Absorbance DNA Quantitation Using BMG LABTECH´s POLARstar Omega Microplate Reader
March 30, 2009
•Application Note: Absorbance DNA Quantitation Using BMG LABTECH´s POLARstar Omega Microplate Reader
By Franka Ganske, BMG LABTECH, Offenburg, Germany
The POLARstar Omega offers easy handling for DNA absorbance measurements by simply selecting a wavelength of 260 nm or by measuring a spectrum which covers the absorbance maximum. Furthermore, with the help of the new data analysis software, MARS, it is possible to determine different nucleic acid concentrations depending on the extinction coefficient.
The ratio of A260/A280 indicates how pure the DNA sample is and it can be measured just as easy and within the same measurement time as A260 alone. A full absorbance spectrum in the range of 220-850 nm helps to identify impurities and it can be measured within one second per well.
The data from the measurement was evaluated using the new data analysis software, MARS, from BMG LABTECH. The average value of the blank measurement was subtracted from the measurements made at each concentration and the results plotted. A linear regression fit was performed on the standard values.
Click Here To Download:•Application Note: Absorbance DNA Quantitation Using BMG LABTECH´s POLARstar Omega Microplate Reader
