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Expression Vector Engineering For Cell Line Development - New Roles For “Old” Sequences
11/12/2008
Expression vector and cell line
engineering is the basis for
expression and industrial
production of biopharmaceuticals.
The ultimate goal
is to obtain clonal cell lines that secrete
the protein of interest with high cellspecific
productivity, and at consistently
high levels over an extended number of
cell generations, allowing for scale-up
and cost-efficient large-scale manufacturing.
Productivity and stability of
expression are thus the prerequisites
for developing commercially viable
processes. By Michel Kobr, Philippe Chattelard, and Markus O. Imhof
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Large-Scale Disposable Shaking Bioreactors
11/7/2008
As disposables become
increasingly important in
biopharmaceutical
manufacturing, more
companies are replacing rigid
stainless steel and glass components
with flexible, single-use plastics.
Mixing tanks, filter assemblies, and
tubing are some types of
components that have successfully
been replaced by disposable
elements for cell-culture production
of high-value compounds. By Keyur Raval, Chao-Min Liu, and Jochen Büchs
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Performance Evaluation Of An Automated Bioreactor Sampling System For Mammalian Cell Cultivation
11/4/2008
Two common goals of biotherapeutics development are reduction in timelines and increased process
understanding. An automated bioreactor sampling system developed by Groton Biosystems has proven its
capacity to address those two seemingly conflicting needs for the industry.
Due to the extreme length and stringent sterility requirement of mammalian cell cultivation, sampling
automation of mammalian bioreactors remains an unmet need. By Groton Biosystems
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White Paper: Performance Evaluation Of An Automated Bioreactor Sampling System For Mammalian Cell Cultivation
11/4/2008
Two common goals of biotherapeutics development are reduction in timelines and increased process
understanding. An automated bioreactor sampling system developed by Groton Biosystems has proven its
capacity to address those two seemingly conflicting needs for the industry.
Due to the extreme length and stringent sterility requirement of mammalian cell cultivation, sampling
automation of mammalian bioreactors remains an unmet need. By Groton Biosystems
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Case Study: IND-Enabling Studies And Chemical Synthesis Completed — Nine Months From Lead Selection
10/1/2008
A client asked Ricerca to develop a robust chemical process, complete GLP toxicology to support a major program, and file an IND — all on a very aggressive timeline. By Ricerca Biosciences, LLC
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IND-Enabling Studies And Chemical Synthesis Completed — Nine Months From Lead Selection
10/1/2008
A client asked Ricerca to develop a robust chemical process, complete GLP toxicology to support a major program, and file an IND — all on a very aggressive timeline. By Ricerca Biosciences, LLC
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Finding The Right Contract Manufacturer
8/18/2008
Finding a suitable contract manufacturer is,
perhaps, one of the most viable options. The pharmaceutical
company can concentrate effort and money
on research and development and marketing, while
the contract manufacturer takes care of the manufacturing
process, including validation, support for license
application and packaging. In addition, outsourcing can
be scheduled into the strategic planning of a drug’s lifecycle.
During the early development phases, which last
years, a partner with the necessary facilities and experience
can be sought out and brought onboard. By Vetter Pharma-Fertigung GmbH & Co. KG
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Poster: Enabling Drug Development And Manufacturing With Innovative Single Use Process Monitoring Technologies
7/21/2008
Single use manufacturing systems for production of bio-therapeutics, where the system arrives to the end-user gamma-irradiated and/or otherwise sterile and where all product contact parts are disposed after use, are in high demand due to the large number of biological products in the pipeline and to the requirement for flexibility in manufacture of products for clinical trials and commercial use
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Disposable Spinner Flasks For Scale Up Or Production Of Proteins
6/11/2008
Suspension cell culture is increasingly being integrated into drug discovery campaigns,
and suitable scale up vessels for suspension growth are evolving to keep
pace. Historically, either disposable plastic shaker flasks or glass spinner vessels
have been used for such culturing
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Article: Molecular Probes® NanoOrange® Assay Performed On BMG LABTECH FLUOstar OPTIMA Microplate Reader
4/1/2008
The fluorescent NanoOrange® Protein Quantitation Kit from Molecular Probes® (Invitrogen) can be easily adapted for use with a microplate reader such as the FLUOstar OPTIMA from BMG LABTECH and used in a high throughput manner. High and small protein concentration range is evaluated. NanoOrange® can also be performed on other BMG LABTECH microplate readers, including the FLUOstar and POLARstar Omegas, as well as the NOVOstar and PHERAstar Plus
